ph catalase experiment

Catalase Activity and pH 2 ml of H 2 O 2 was added to five homogenate solutions with varying pHs: 1 (acidic), 3 (acidic), 7 (neutral), 9 (basic), and 12 (basic). Summary: Reaction is fastest at neutral to basic pHs. Catalase is adapted to work best under cellular conditions. Most cells have a pH around 7, and our reaction worked well at that pH.

Cells make the enzyme catalase to remove hydrogen peroxide. This investigation looks at the rate of oxygen production by the catalase in pureed potato as the concentration of hydrogen peroxide varies. The oxygen produced in 30 seconds is collected over water. Then the rate of reaction is calculated.

1. How does pH affect the reaction rate of catalase? Propose a way to refine your experiment to find the exact, or OPTIMAL pH and temperature of catalase. 2. The following graph shows reaction rates of various enzymes in the body. Pepsin is found in the stomach, amylase in the saliva, and phosphatase in the liver.

A common enzyme lab for students to measure the impact of temperature and pH on the efficiency of catalase. Catalase is an enzyme is found in almost all living organisms that breaks down hydrogen peroxide (H2O2) into oxygen and water. Many teachers use raw chicken liver or potato as the source of the catalase.

This experiment investigates the effect of pH on catalase activity. and observes the catalytic action of two sources of catalase on hydrogen peroxide. Hypotheses . Hypothesis 1: If the pH is below or above the optimum pH, the rate of the catalytic action of catalase will decrease. Hypothesis 2:Animal cells contain more catalase than plant cells.

7 Lab 6. Enzymes – Catalase Function Lab 6: Enzymes – Catalase Function OBJECTIVES. Practice and apply hypothesis testing. Practice experimental design. Gain a better understanding of enzymes and some conditions that affect enzyme activity and the rate of an enzyme-catalyzed reaction.

Repeat the catalase reaction but this time vary conditions such as the pH by adding vinegar or baking soda, or change the reaction temperature by heating the solution in the microwave. Can you identify which conditions are optimal for the catalase reaction? Are there any conditions that eliminate the catalase activity?

Yeast contains an enzyme, called catalase, that acts as a catalyst for the reaction that breaks down hydrogen peroxide into oxygen and water (2H 2 O 2 2H 2 O + O 2). Safety note: oxygen is a highly reactive gas, adult supervision recommended for this project. For your background research, be sure that you understand substrate, catalyst ...

Figure 1. When blended liver is exposed to hydrogen peroxide, the catalase enzyme in the liver reacts with the hydrogen peroxide to form oxygen gas, which creates the visible bubbles in this photograph, and water. In this science fair project, you will use fresh (or frozen) liver as a source of catalase and investigate how the activity of the ...

The enzyme used in this experiment is catalase. The substrate of the enzyme is hydrogen peroxide. The dependent variable in this experiment is the volume of oxygen gas produced. The optimal pH range for catalase is around pH 7. The activity of catalase increases as the pH increases up to its optimal pH range, after which activity decreases.