define experiment in chemistry

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Experiment Definition in Science – What Is a Science Experiment?

In science, an experiment is simply a test of a hypothesis in the scientific method . It is a controlled examination of cause and effect. Here is a look at what a science experiment is (and is not), the key factors in an experiment, examples, and types of experiments.

Experiment Definition in Science

By definition, an experiment is a procedure that tests a hypothesis. A hypothesis, in turn, is a prediction of cause and effect or the predicted outcome of changing one factor of a situation. Both the hypothesis and experiment are components of the scientific method. The steps of the scientific method are:

• Make observations.
• Ask a question or identify a problem.
• State a hypothesis.
• Perform an experiment that tests the hypothesis.
• Based on the results of the experiment, either accept or reject the hypothesis.
• Draw conclusions and report the outcome of the experiment.

Key Parts of an Experiment

The two key parts of an experiment are the independent and dependent variables. The independent variable is the one factor that you control or change in an experiment. The dependent variable is the factor that you measure that responds to the independent variable. An experiment often includes other types of variables , but at its heart, it’s all about the relationship between the independent and dependent variable.

Examples of Experiments

Fertilizer and plant size.

For example, you think a certain fertilizer helps plants grow better. You’ve watched your plants grow and they seem to do better when they have the fertilizer compared to when they don’t. But, observations are only the beginning of science. So, you state a hypothesis: Adding fertilizer increases plant size. Note, you could have stated the hypothesis in different ways. Maybe you think the fertilizer increases plant mass or fruit production, for example. However you state the hypothesis, it includes both the independent and dependent variables. In this case, the independent variable is the presence or absence of fertilizer. The dependent variable is the response to the independent variable, which is the size of the plants.

Now that you have a hypothesis, the next step is designing an experiment that tests it. Experimental design is very important because the way you conduct an experiment influences its outcome. For example, if you use too small of an amount of fertilizer you may see no effect from the treatment. Or, if you dump an entire container of fertilizer on a plant you could kill it! So, recording the steps of the experiment help you judge the outcome of the experiment and aid others who come after you and examine your work. Other factors that might influence your results might include the species of plant and duration of the treatment. Record any conditions that might affect the outcome. Ideally, you want the only difference between your two groups of plants to be whether or not they receive fertilizer. Then, measure the height of the plants and see if there is a difference between the two groups.

Salt and Cookies

You don’t need a lab for an experiment. For example, consider a baking experiment. Let’s say you like the flavor of salt in your cookies, but you’re pretty sure the batch you made using extra salt fell a bit flat. If you double the amount of salt in a recipe, will it affect their size? Here, the independent variable is the amount of salt in the recipe and the dependent variable is cookie size.

Test this hypothesis with an experiment. Bake cookies using the normal recipe (your control group ) and bake some using twice the salt (the experimental group). Make sure it’s the exact same recipe. Bake the cookies at the same temperature and for the same time. Only change the amount of salt in the recipe. Then measure the height or diameter of the cookies and decide whether to accept or reject the hypothesis.

Examples of Things That Are Not Experiments

Based on the examples of experiments, you should see what is not an experiment:

• Making observations does not constitute an experiment. Initial observations often lead to an experiment, but are not a substitute for one.
• Making a model is not an experiment.
• Neither is making a poster.
• Just trying something to see what happens is not an experiment. You need a hypothesis or prediction about the outcome.
• Changing a lot of things at once isn’t an experiment. You only have one independent and one dependent variable. However, in an experiment, you might suspect the independent variable has an effect on a separate. So, you design a new experiment to test this.

Types of Experiments

There are three main types of experiments: controlled experiments, natural experiments, and field experiments,

• Controlled experiment : A controlled experiment compares two groups of samples that differ only in independent variable. For example, a drug trial compares the effect of a group taking a placebo (control group) against those getting the drug (the treatment group). Experiments in a lab or home generally are controlled experiments
• Natural experiment : Another name for a natural experiment is a quasi-experiment. In this type of experiment, the researcher does not directly control the independent variable, plus there may be other variables at play. Here, the goal is establishing a correlation between the independent and dependent variable. For example, in the formation of new elements a scientist hypothesizes that a certain collision between particles creates a new atom. But, other outcomes may be possible. Or, perhaps only decay products are observed that indicate the element, and not the new atom itself. Many fields of science rely on natural experiments, since controlled experiments aren’t always possible.
• Field experiment : While a controlled experiments takes place in a lab or other controlled setting, a field experiment occurs in a natural setting. Some phenomena cannot be readily studied in a lab or else the setting exerts an influence that affects the results. So, a field experiment may have higher validity. However, since the setting is not controlled, it is also subject to external factors and potential contamination. For example, if you study whether a certain plumage color affects bird mate selection, a field experiment in a natural environment eliminates the stressors of an artificial environment. Yet, other factors that could be controlled in a lab may influence results. For example, nutrition and health are controlled in a lab, but not in the field.
• Bailey, R.A. (2008). Design of Comparative Experiments . Cambridge: Cambridge University Press. ISBN 9780521683579.
• di Francia, G. Toraldo (1981). The Investigation of the Physical World . Cambridge University Press. ISBN 0-521-29925-X.
• Hinkelmann, Klaus; Kempthorne, Oscar (2008). Design and Analysis of Experiments. Volume I: Introduction to Experimental Design (2nd ed.). Wiley. ISBN 978-0-471-72756-9.
• Holland, Paul W. (December 1986). “Statistics and Causal Inference”.  Journal of the American Statistical Association . 81 (396): 945–960. doi: 10.2307/2289064
• Stohr-Hunt, Patricia (1996). “An Analysis of Frequency of Hands-on Experience and Science Achievement”. Journal of Research in Science Teaching . 33 (1): 101–109. doi: 10.1002/(SICI)1098-2736(199601)33:1<101::AID-TEA6>3.0.CO;2-Z

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Definition of experiment

 (Entry 1 of 2)

Definition of experiment  (Entry 2 of 2)

intransitive verb

• experimentation

Examples of experiment in a Sentence

These examples are programmatically compiled from various online sources to illustrate current usage of the word 'experiment.' Any opinions expressed in the examples do not represent those of Merriam-Webster or its editors. Send us feedback about these examples.

Word History

Middle English, "testing, proof, remedy," borrowed from Anglo-French esperiment, borrowed from Latin experīmentum "testing, experience, proof," from experīrī "to put to the test, attempt, have experience of, undergo" + -mentum -ment — more at experience entry 1

verbal derivative of experiment entry 1

14th century, in the meaning defined at sense 1a

1787, in the meaning defined above

Phrases Containing experiment

• control experiment
• controlled experiment
• experiment station
• pre - experiment
• thought experiment

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Cite this Entry

“Experiment.” Merriam-Webster.com Dictionary , Merriam-Webster, https://www.merriam-webster.com/dictionary/experiment. Accessed 12 Sep. 2024.

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Definitions of Control, Constant, Independent and Dependent Variables in a Science Experiment

Why Should You Only Test for One Variable at a Time in an Experiment?

The point of an experiment is to help define the cause and effect relationships between components of a natural process or reaction. The factors that can change value during an experiment or between experiments, such as water temperature, are called scientific variables, while those that stay the same, such as acceleration due to gravity at a certain location, are called constants.

The scientific method includes three main types of variables: constants, independent, and dependent variables. In a science experiment, each of these variables define a different measured or constrained aspect of the system.

Constant Variables

Experimental constants are values that should not change either during or between experiments. Many natural forces and properties, such as the speed of light and the atomic weight of gold, are experimental constants. In some cases, a property can be considered constant for the purposes of an experiment even though it technically could change under certain circumstances. The boiling point of water changes with altitude and acceleration due to gravity decreases with distance from the earth, but for experiments in one location these can also be considered constants.

Sometimes also called a controlled variable. A constant is a variable that could change, but that the experimenter intentionally keeps constant in order to more clearly isolate the relationship between the independent variable and the dependent variable.

If extraneous variables are not properly constrained, they are referred to as confounding variables, as they interfere with the interpretation of the results of the experiment.

Some examples of control variables might be found with an experiment examining the relationship between the amount of sunlight plants receive (independent variable) and subsequent plant growth (dependent variable). The experiment should control the amount of water the plants receive and when, what type of soil they are planted in, the type of plant, and as many other different variables as possible. This way, only the amount of light is being changed between trials, and the outcome of the experiment can be directly applied to understanding only this relationship.

Independent Variable

The independent variable in an experiment is the variable whose value the scientist systematically changes in order to see what effect the changes have. A well-designed experiment has only one independent variable in order to maintain a fair test. If the experimenter were to change two or more variables, it would be harder to explain what caused the changes in the experimental results. For example, someone trying to find how quickly water boils could alter the volume of water or the heating temperature, but not both.

Dependent Variable

A dependent variable – sometimes called a responding variable – is what the experimenter observes to find the effect of systematically varying the independent variable. While an experiment may have multiple dependent variables, it is often wisest to focus the experiment on one dependent variable so that the relationship between it and the independent variable can be clearly isolated. For example, an experiment could examine how much sugar can dissolve in a set volume of water at various temperatures. The experimenter systematically alters temperature (independent variable) to see its effect on the quantity of dissolved sugar (dependent variable).

Control Groups

In some experiment designs, there might be one effect or manipulated variable that is being measured. Sometimes there might be one collection of measurements or subjects completely separated from this variable called the control group. These control groups are held as a standard to measure the results of a scientific experiment.

An example of such a situation might be a study regarding the effectiveness of a certain medication. There might be multiple experimental groups that receive the medication in varying doses and applications, and there would likely be a control group that does not receive the medication at all.

Representing Results

Identifying which variables are independent, dependent, and controlled helps to collect data, perform useful experiments, and accurately communicate results. When graphing or displaying data, it is crucial to represent data accurately and understandably. Typically, the independent variable goes on the x-axis, and the dependent variable goes on the y-axis.

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• A chemical reaction is a process in which one or more substances, also called reactants, are converted to one or more different substances, known as products. Substances are either chemical elements or compounds .
• A chemical reaction rearranges the constituent atoms of the reactants to create different substances as products. The properties of the products are different from those of the reactants.
• Chemical reactions differ from physical changes, which include changes of state, such as ice melting to water and water evaporating to vapor. If a physical change occurs, the physical properties of a substance will change, but its chemical identity will remain the same.

According to the modern view of chemical reactions, bonds between atoms in the reactants must be broken, and the atoms or pieces of molecules are reassembled into products by forming new bonds. Energy is absorbed to break bonds, and energy is evolved as bonds are made. In some reactions the energy required to break bonds is larger than the energy evolved in making new bonds, and the net result is the absorption of energy. Hence, different types of bonds may be formed in a reaction. A Lewis acid-base reaction , for example, involves the formation of a covalent bond between a Lewis base, a species that supplies an electron pair, and a Lewis acid, a species that can accept an electron pair. Ammonia is an example of a Lewis base. A pair of electrons located on a nitrogen atom may be used to form a chemical bond to a Lewis acid.

Chemists classify chemical reactions in a number of ways: by type of product, by types of reactants, by reaction outcome, and by reaction mechanism. Often a given reaction can be placed in two or even three categories, including gas -forming and precipitation reactions. Many reactions produce a gas such as carbon dioxide , hydrogen sulfide , ammonia , or sulfur dioxide . Cake batter rising is caused by a gas-forming reaction between an acid and baking soda (sodium hydrogen carbonate). Classification by types of reactants include acid-base reactions and oxidation-reduction reactions , which involve the transfer of one or more electrons from a reducing agent to an oxidizing agent. Examples of classification by reaction outcome include decomposition, polymerization , substitution , and elimination and addition reactions. Chain reactions and photolysis reactions are examples of classification by reaction mechanism, which provides details on how atoms are shuffled and reassembled in the formation of products.

chemical reaction , a process in which one or more substances, the reactants , are converted to one or more different substances, the products. Substances are either chemical elements or compounds . A chemical reaction rearranges the constituent atoms of the reactants to create different substances as products.

Chemical reactions are an integral part of technology, of culture , and indeed of life itself. Burning fuels, smelting iron , making glass and pottery , brewing beer , and making wine and cheese are among many examples of activities incorporating chemical reactions that have been known and used for thousands of years. Chemical reactions abound in the geology of Earth , in the atmosphere and oceans , and in a vast array of complicated processes that occur in all living systems.

Chemical reactions must be distinguished from physical changes. Physical changes include changes of state, such as ice melting to water and water evaporating to vapour. If a physical change occurs, the physical properties of a substance will change, but its chemical identity will remain the same. No matter what its physical state, water (H 2 O) is the same compound , with each molecule composed of two atoms of hydrogen and one atom of oxygen . However, if water, as ice, liquid, or vapour, encounters sodium metal (Na), the atoms will be redistributed to give the new substances molecular hydrogen (H 2 ) and sodium hydroxide (NaOH). By this, we know that a chemical change or reaction has occurred.

The concept of a chemical reaction dates back about 250 years. It had its origins in early experiments that classified substances as elements and compounds and in theories that explained these processes. Development of the concept of a chemical reaction had a primary role in defining the science of chemistry as it is known today.

The first substantive studies in this area were on gases . The identification of oxygen in the 18th century by Swedish chemist Carl Wilhelm Scheele and English clergyman Joseph Priestley had particular significance. The influence of French chemist Antoine-Laurent Lavoisier was especially notable, in that his insights confirmed the importance of quantitative measurements of chemical processes. In his book Traité élémentaire de chimie (1789; Elementary Treatise on Chemistry ), Lavoisier identified 33 “elements”—substances not broken down into simpler entities. Among his many discoveries, Lavoisier accurately measured the weight gained when elements were oxidized, and he ascribed the result to the combining of the element with oxygen . The concept of chemical reactions involving the combination of elements clearly emerged from his writing, and his approach led others to pursue experimental chemistry as a quantitative science.

The other occurrence of historical significance concerning chemical reactions was the development of atomic theory . For this, much credit goes to English chemist John Dalton , who postulated his atomic theory early in the 19th century. Dalton maintained that matter is composed of small, indivisible particles, that the particles, or atoms , of each element were unique, and that chemical reactions were involved in rearranging atoms to form new substances. This view of chemical reactions accurately defines the current subject. Dalton’s theory provided a basis for understanding the results of earlier experimentalists, including the law of conservation of matter (matter is neither created nor destroyed) and the law of constant composition (all samples of a substance have identical elemental compositions).

Thus, experiment and theory, the two cornerstones of chemical science in the modern world, together defined the concept of chemical reactions. Today experimental chemistry provides innumerable examples, and theoretical chemistry allows an understanding of their meaning.

Basic concepts of chemical reactions

When making a new substance from other substances, chemists say either that they carry out a synthesis or that they synthesize the new material. Reactants are converted to products, and the process is symbolized by a chemical equation . For example, iron (Fe) and sulfur (S) combine to form iron sulfide (FeS). Fe(s) + S(s) → FeS(s) The plus sign indicates that iron reacts with sulfur. The arrow signifies that the reaction “forms” or “yields” iron sulfide, the product. The state of matter of reactants and products is designated with the symbols (s) for solids , (l) for liquids , and (g) for gases .

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• Guide to Experimental Design | Overview, Steps, & Examples

Guide to Experimental Design | Overview, 5 steps & Examples

Published on December 3, 2019 by Rebecca Bevans . Revised on June 21, 2023.

Experiments are used to study causal relationships . You manipulate one or more independent variables and measure their effect on one or more dependent variables.

Experimental design create a set of procedures to systematically test a hypothesis . A good experimental design requires a strong understanding of the system you are studying.

There are five key steps in designing an experiment:

• Consider your variables and how they are related
• Write a specific, testable hypothesis
• Design experimental treatments to manipulate your independent variable
• Assign subjects to groups, either between-subjects or within-subjects
• Plan how you will measure your dependent variable

For valid conclusions, you also need to select a representative sample and control any  extraneous variables that might influence your results. If random assignment of participants to control and treatment groups is impossible, unethical, or highly difficult, consider an observational study instead. This minimizes several types of research bias, particularly sampling bias , survivorship bias , and attrition bias as time passes.

Table of contents

Step 1: define your variables, step 2: write your hypothesis, step 3: design your experimental treatments, step 4: assign your subjects to treatment groups, step 5: measure your dependent variable, other interesting articles, frequently asked questions about experiments.

You should begin with a specific research question . We will work with two research question examples, one from health sciences and one from ecology:

To translate your research question into an experimental hypothesis, you need to define the main variables and make predictions about how they are related.

Start by simply listing the independent and dependent variables .

Then you need to think about possible extraneous and confounding variables and consider how you might control  them in your experiment.

Finally, you can put these variables together into a diagram. Use arrows to show the possible relationships between variables and include signs to show the expected direction of the relationships.

Here we predict that increasing temperature will increase soil respiration and decrease soil moisture, while decreasing soil moisture will lead to decreased soil respiration.

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Now that you have a strong conceptual understanding of the system you are studying, you should be able to write a specific, testable hypothesis that addresses your research question.

The next steps will describe how to design a controlled experiment . In a controlled experiment, you must be able to:

• Systematically and precisely manipulate the independent variable(s).
• Precisely measure the dependent variable(s).
• Control any potential confounding variables.

If your study system doesn’t match these criteria, there are other types of research you can use to answer your research question.

How you manipulate the independent variable can affect the experiment’s external validity – that is, the extent to which the results can be generalized and applied to the broader world.

First, you may need to decide how widely to vary your independent variable.

• just slightly above the natural range for your study region.
• over a wider range of temperatures to mimic future warming.
• over an extreme range that is beyond any possible natural variation.

Second, you may need to choose how finely to vary your independent variable. Sometimes this choice is made for you by your experimental system, but often you will need to decide, and this will affect how much you can infer from your results.

• a categorical variable : either as binary (yes/no) or as levels of a factor (no phone use, low phone use, high phone use).
• a continuous variable (minutes of phone use measured every night).

How you apply your experimental treatments to your test subjects is crucial for obtaining valid and reliable results.

First, you need to consider the study size : how many individuals will be included in the experiment? In general, the more subjects you include, the greater your experiment’s statistical power , which determines how much confidence you can have in your results.

Then you need to randomly assign your subjects to treatment groups . Each group receives a different level of the treatment (e.g. no phone use, low phone use, high phone use).

You should also include a control group , which receives no treatment. The control group tells us what would have happened to your test subjects without any experimental intervention.

When assigning your subjects to groups, there are two main choices you need to make:

• A completely randomized design vs a randomized block design .
• A between-subjects design vs a within-subjects design .

Randomization

An experiment can be completely randomized or randomized within blocks (aka strata):

• In a completely randomized design , every subject is assigned to a treatment group at random.
• In a randomized block design (aka stratified random design), subjects are first grouped according to a characteristic they share, and then randomly assigned to treatments within those groups.

Sometimes randomization isn’t practical or ethical , so researchers create partially-random or even non-random designs. An experimental design where treatments aren’t randomly assigned is called a quasi-experimental design .

Between-subjects vs. within-subjects

In a between-subjects design (also known as an independent measures design or classic ANOVA design), individuals receive only one of the possible levels of an experimental treatment.

In medical or social research, you might also use matched pairs within your between-subjects design to make sure that each treatment group contains the same variety of test subjects in the same proportions.

In a within-subjects design (also known as a repeated measures design), every individual receives each of the experimental treatments consecutively, and their responses to each treatment are measured.

Within-subjects or repeated measures can also refer to an experimental design where an effect emerges over time, and individual responses are measured over time in order to measure this effect as it emerges.

Counterbalancing (randomizing or reversing the order of treatments among subjects) is often used in within-subjects designs to ensure that the order of treatment application doesn’t influence the results of the experiment.

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Finally, you need to decide how you’ll collect data on your dependent variable outcomes. You should aim for reliable and valid measurements that minimize research bias or error.

Some variables, like temperature, can be objectively measured with scientific instruments. Others may need to be operationalized to turn them into measurable observations.

• Ask participants to record what time they go to sleep and get up each day.
• Ask participants to wear a sleep tracker.

How precisely you measure your dependent variable also affects the kinds of statistical analysis you can use on your data.

Experiments are always context-dependent, and a good experimental design will take into account all of the unique considerations of your study system to produce information that is both valid and relevant to your research question.

If you want to know more about statistics , methodology , or research bias , make sure to check out some of our other articles with explanations and examples.

• Student’s  t -distribution
• Normal distribution
• Null and Alternative Hypotheses
• Chi square tests
• Confidence interval
• Cluster sampling
• Stratified sampling
• Data cleansing
• Reproducibility vs Replicability
• Peer review
• Likert scale

Research bias

• Implicit bias
• Framing effect
• Cognitive bias
• Placebo effect
• Hawthorne effect
• Hindsight bias
• Affect heuristic

Experimental design means planning a set of procedures to investigate a relationship between variables . To design a controlled experiment, you need:

• A testable hypothesis
• At least one independent variable that can be precisely manipulated
• At least one dependent variable that can be precisely measured

When designing the experiment, you decide:

• How you will manipulate the variable(s)
• How you will control for any potential confounding variables
• How many subjects or samples will be included in the study
• How subjects will be assigned to treatment levels

Experimental design is essential to the internal and external validity of your experiment.

The key difference between observational studies and experimental designs is that a well-done observational study does not influence the responses of participants, while experiments do have some sort of treatment condition applied to at least some participants by random assignment .

A confounding variable , also called a confounder or confounding factor, is a third variable in a study examining a potential cause-and-effect relationship.

A confounding variable is related to both the supposed cause and the supposed effect of the study. It can be difficult to separate the true effect of the independent variable from the effect of the confounding variable.

In your research design , it’s important to identify potential confounding variables and plan how you will reduce their impact.

In a between-subjects design , every participant experiences only one condition, and researchers assess group differences between participants in various conditions.

In a within-subjects design , each participant experiences all conditions, and researchers test the same participants repeatedly for differences between conditions.

The word “between” means that you’re comparing different conditions between groups, while the word “within” means you’re comparing different conditions within the same group.

An experimental group, also known as a treatment group, receives the treatment whose effect researchers wish to study, whereas a control group does not. They should be identical in all other ways.

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What Is a Variable in Science?

Understanding Variables in a Science Experiment

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• Ph.D., Biomedical Sciences, University of Tennessee at Knoxville
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Variables are an important part of science projects and experiments. What is a variable? Basically, a variable is any factor that can be controlled, changed, or measured in an experiment. Scientific experiments have several types of variables. The independent and dependent variables are the ones usually plotted on a chart or graph, but there are other types of variables you may encounter.

Types of Variables

• Independent Variable: The independent variable is the one condition that you change in an experiment. Example: In an experiment measuring the effect of temperature on solubility, the independent variable is temperature.
• Dependent Variable: The dependent variable is the variable that you measure or observe. The dependent variable gets its name because it is the factor that is dependent on the state of the independent variable . Example: In the experiment measuring the effect of temperature on solubility, solubility would be the dependent variable.
• Controlled Variable: A controlled variable or constant variable is a variable that does not change during an experiment. Example : In the experiment measuring the effect of temperature on solubility, controlled variable could include the source of water used in the experiment, the size and type of containers used to mix chemicals, and the amount of mixing time allowed for each solution.
• Extraneous Variables: Extraneous variables are "extra" variables that may influence the outcome of an experiment but aren't taken into account during measurement. Ideally, these variables won't impact the final conclusion drawn by the experiment, but they may introduce error into scientific results. If you are aware of any extraneous variables, you should enter them in your lab notebook . Examples of extraneous variables include accidents, factors you either can't control or can't measure, and factors you consider unimportant. Every experiment has extraneous variables. Example : You are conducting an experiment to see which paper airplane design flies longest. You may consider the color of the paper to be an extraneous variable. You note in your lab book that different colors of papers were used. Ideally, this variable does not affect your outcome.

Using Variables in Science Experiment

In a science experiment , only one variable is changed at a time (the independent variable) to test how this changes the dependent variable. The researcher may measure other factors that either remain constant or change during the course of the experiment but are not believed to affect its outcome. These are controlled variables. Any other factors that might be changed if someone else conducted the experiment but seemed unimportant should also be noted. Also, any accidents that occur should be recorded. These are extraneous variables.

Variables and Attributes

In science, when a variable is studied, its attribute is recorded. A variable is a characteristic, while an attribute is its state. For example, if eye color is the variable, its attribute might be green, brown, or blue. If height is the variable, its attribute might be 5 m, 2.5 cm, or 1.22 km.

• Earl R. Babbie. The Practice of Social Research , 12th edition. Wadsworth Publishing, 2009.
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